Hypercarb is a unique material for HPLC manufactured by Thermo Fisher Scientific Hypersil-Keystone. Hypercarb consists of fully porous spherical carbon particles comprised of flat sheets of hexagonally arranged carbon atoms. The carbon atoms have a fully satisfied valence and offer completely different retention and selectivity to silica and polymer based phases.
Hypercarb can be used for RP and NP chromatography with a pH range from 1 to 14, and at extremes of temperature. Hypercarb excels in the analysis of structurally related components (e.g. stereo- and diastereoisomers and positional isomers) where no separation occurs on conventional materials.
Hypercarb retains polar compounds via a 'polar retention effect' where a stronger analyte-sorbent attraction occurs as the polarity of the analyte increases, so the more polar the analyte the longer it's retention time compared to silica-based phases. Due to the unique retention mechanism of Hypercarb, we recommend that you do not use a column longer than 100mm or 150mm in length. Please contact us for technical assistance.